Key result
Novel BVDV strain SH9/11 shares ~93% identity with classical strains despite unique genetic and antibody variations.
Why the study?
Distinct bovine viral diarrhea virus strains might exist among free-ranging roe deer in Germany, but their characterization was lacking.
Distinct BVDV strains might exist among free-ranging roe deer in Germany.
May indicate wildlife BVDV reservoirs in roe deer; leaves open cross-species transmission risks and need for targeted surveillance.
The 5' untranslated region (5' UTR) of cytopathogenic pestiviruses isolated from two seronegative roe deer (Capreolus capreolus) in northern Germany was partially sequenced and compared with those of 28 other pestiviruses. Due to the occurrence within a narrow location and the complete identity of the sequenced fragments from both roe deer isolates (SH9 and SH11) they seem to belong to the same bovine virus diarrhea virus (BVDV) strain called SH9/11. This strain is highly homologous (up to 93% identity) to "classical" BVDV strains. However, SH9/11 has characteristic variations in its 5' UTR distinct from all other pestiviruses analyzed in this study. Strain SH9/11 is more similar to BVDV group I than to group II, although it is clearly separated from all other cattle isolates tested. In monoclonal antibody (mAb)-typing studies, isolate SH9 reacted with one pestivirus-specific mAb (C16), with two BVDV specific mAbs (N2B12 and D5), and with one mAb (f48) raised against the E2 protein of classical swine fever virus out of a panel of 13 mAbs. The separate position of strain SH9/11 again was demonstrated by the unique reaction pattern of isolate SH9 when compared with other mAb f48-positive BVDV and BDV strains. All these results indicate that indicate that distinct BVDV strains might exist among freeranging roe deer in Germany.
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Fischer et al. (1998) studied Bovine viral diarrhea virus (BVDV) infection (n=2). BVDV strain SH9/11 isolation and characterization vs. 28 other pestiviruses was evaluated on Genetic and antigenic characterization of the 5' UTR and mAb-typing. A novel BVDV strain (SH9/11) isolated from roe deer in Germany showed up to 93% identity to classical BVDV strains but possessed characteristic 5' UTR variations and a unique mAb reaction pattern.
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