What question did this study set out to answer?

To identify the mechanism governing SMC-mediated chromosomal arm alignment in Caulobacter crescentus and assess how transcription impacts this process.

August 1, 2017Cell ReportsOpen Access

SMC Progressively Aligns Chromosomal Arms in Caulobacter crescentus but Is Antagonized by Convergent Transcription

View Full Paper

Ask AI

Bookmark

Authors

NTNgat T. TranJohn Innes Centre MLMichael T. LaubHoward Hughes Medical Institute TLTung B. K. LeJohn Innes Centre

Discussion

Member takes

Implication

Mechanistic study reveals that SMC aligns chromosomal arms in Caulobacter crescentus, indicating that head-on transcription directly antagonizes SMC translocation and genome organization.

Key Points

To identify the mechanism governing SMC-mediated chromosomal arm alignment in Caulobacter crescentus and assess how transcription impacts this process.
Conducted genome-wide chromosome conformation analysis and single-cell microscopy in Caulobacter crescentus.
Investigated the requirement of centromeric parS loading sites and the chromosome-partitioning protein ParB for SMC recruitment.
Assessed chromosomal arm alignment across genomic regions with highly transcribed genes oriented either co-directionally or head-on to SMC translocation.
Caulobacter SMC recruitment to the centromeric parS site and subsequent chromosomal arm alignment require the partitioning protein ParB.
SMC tethers parS-proximal regions of the left and right chromosomal arms together, progressively driving arm alignment down the cell.
Highly transcribed genes oriented head-on against SMC translocation disrupt chromosomal arm alignment, indicating that convergent transcription directly impedes SMC translocation.

Cite This Study

Tran et al. (2017) studied this question.

synapsesocial.com/papers/6aaa0ea9d33e8d4647558274 https://doi.org/10.1016/j.celrep.2017.08.026

View Full Paper

Ask AI

Bookmark

Implication

Mechanistic study reveals that SMC aligns chromosomal arms in Caulobacter crescentus, indicating that head-on transcription directly antagonizes SMC translocation and genome organization.

SMC Progressively Aligns Chromosomal Arms in Caulobacter crescentus but Is Antagonized by Convergent Transcription

Discussion

Member takes

Implication

Key Points

Cite This Study

SMC Progressively Aligns Chromosomal Arms in Caulobacter crescentus but Is Antagonized by Convergent Transcription

Discussion

Member takes

Implication

Key Points

Cite This Study

Also Consider

Also Consider