The detection of oxidized bases in cellular DNA is a challenging problem due to the sensitivity required that should be high enough in order to detect one modification per 106 DNA bases. In this respect, gas chromatography mass spectrometry (GC-MS) has been extensively used during the last ten years. However, comparison of the results obtained by this method and those inferred from high performance liquid chromatography analyses coupled with an electrochemical detection (HPLC-EC) indicated an overestimation in the level of the oxidized DNA bases in the GC-MS
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Ravanat et al. (1997) studied this question.