Key result
CRISPR genome editing provides a robust tool to investigate viral life cycles and advance diagnostics.
Why the study?
Understanding the dynamic relationship between viral pathogens and cellular host factors is critical for understanding viral replication, disease mechanisms, and anti-viral therapeutic development.
CRISPR genome editing technology provides robust tools for investigating viral life cycles and developing diagnostics for emerging viral pathogens like SARS-CoV-2.
CRISPR screens map SARS-CoV-2 host factors; leaves open validation for cardiovascular complications.
Understanding the dynamic relationship between viral pathogens and cellular host factors is critical to furthering our knowledge of viral replication, disease mechanisms and development of anti-viral therapeutics. CRISPR genome editing technology has enhanced this understanding, by allowing identification of pro-viral and anti-viral cellular host factors for a wide range of viruses, most recently the cause of the COVID-19 pandemic, SARS-CoV-2. This review will discuss how CRISPR knockout and CRISPR activation genome-wide screening methods are a robust tool to investigate the viral life cycle and how other class 2 CRISPR systems are being repurposed for diagnostics.
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Kirby et al. (2021) conducted a review in Viral pathogens. CRISPR genome editing technology was evaluated. CRISPR genome editing technology serves as a robust tool to investigate viral life cycles and is being repurposed for viral diagnostics.
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