Romil (England).Water for chromatography was purchased from Merck (Germany).Mobile phase was filtered using 0.45 µm nylon membrane filter (UK). Equipment and chromatographic conditionsThe analysis of drugs was carried out on a Shimadzu LC-20 XR, prominence (Kyoto, Japan) is equipped with an auto sampler (SIL-20AC XR, Shimadzu, Japan) and PDA detector (SPD-M20A, Japan) was used for the analysis.Peak areas were integrated using a Shimadzu LC solution (version 5.41.240) software program.The data was recorded using LC-solution software.A NSXX sonics ultrasonic bath (NS-A-12-7H, Germany) was used for degassing of the mobile phase.Experimental conditions were optimized on a ACE C18/CN column (100 x 4.6 mm, 5 µm) at a temperature of 25ºC with column oven (CTO-20AC) and the flow rate of the mobile phase was 1.5 ml min-1.
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