In Ovulo Embryo Development and Plant Formation from Stenospermic Genotypes of Vitis Vinifera

Embryo enlargement and plant formation via in ovulo embryo culture was studied in stenospermic grapes. Genotypes, culture media and their interactions significantly affected embryo enlargement, whereas culture dates did not. Genotype P60-58, when compared to cv. Thompson Seedless, had the greatest number of enlarged embryos after 3 months in culture. P60-58 ovules cultured on Cain's basal medium plus L-serine or L-glutamine and cv. Thompson Seedless ovules cultured on Cain's basal medium plus L-cysteine or L-asparagine produced the greatest number of enlarged embryos. Viable embryos and morphologically normal-appearing plants were obtained. Polyembryony was observed in cultured zygotic embryos.