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March 1, 2000Journal of Clinical MicrobiologyOpen Access

Growth of Cowdria ruminantium , the Causative Agent of Heartwater, in a Tick Cell Line

Lesley Bell‐Sakyi Edith Paxton Ulrike G. Munderloh Keith Sumption

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Abstract

The tick-borne rickettsia Cowdria ruminantium has been propagated continuously for over 500 days in the Ixodes scapularis tick cell line IDE8 by using the Gardel isolate from bovine endothelial cells as an inoculum. Infection of the tick cells was confirmed by PCR, karyotyping, electron microscopy, and reinfection of bovine cells.

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Bell‐Sakyi et al. (2000) studied this question.

synapsesocial.com/papers/6aaa5c04c85bdfeaa654ccc9 https://doi.org/10.1128/jcm.38.3.1238-1240.2000

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