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Long non-coding RNAs (lncRNAs) are dysregulated in various cancers.However, the clinical relevance and functional roles of AFAP1-AS1 in colon cancer (CC) have not been clarified.We analyzed the lncRNA expression patterns in Gene Expression Omnibus (GEO) datasets and the Cancer Genome Atlas (TCGA) RNA-seq datasets, and found that the expression level of AFAP1-AS1 was significantly elevated in CC tissues.High levels of AFAP1-AS1 were associated with poor disease-free survival and overall survival in CC patients.In vitro experiments demonstrated that AFAP1-AS1 knockdown significantly inhibited the cell invasive and migration capability in CC cell line HT-29.AFAP1-AS1 knockdown also increased the expression of E-cadherin and ZO-1 while inhibited the expression of Vimentin, MMP9, ZEB1 and β-catenin, suggesting that AFAP1-AS1 is involved in the epithelial-mesenchymal transition (EMT) process of CC.Further studies confirmed that AFAP1-AS1 knockdown also affected the actin-cytokeratin signaling pathway.Thus, AFAP1-AS1 might be a potential novel diagnostic marker and therapeutic target for CC.
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Bo et al. (2018) studied this question.
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