What question did this study set out to answer?

To assess longitudinal changes in antibiotic susceptibility, serotype distribution, sequence types, and penicillin-binding protein 3 (PBP3) mutations among invasive Haemophilus influenzae isolates in Canada.

December 13, 2016Journal of Antimicrobial ChemotherapyOpen Access

Antibiotic susceptibility and molecular analysis of invasiveHaemophilus influenzaein Canada, 2007 to 2014

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Authors

RTRaymond S. W. TsangUniversity of Alabama MSMichelle ShuelPublic Health Agency of Canada KWKathleen WhytePublic Health Agency of Canada

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Implication

Surveillance study reveals rising ampicillin resistance and penicillin-binding protein mutations in invasive Haemophilus influenzae, highlighting emerging drug resistance in non-typeable strains.

Key Points

To assess longitudinal changes in antibiotic susceptibility, serotype distribution, sequence types, and penicillin-binding protein 3 (PBP3) mutations among invasive Haemophilus influenzae isolates in Canada.
Characterized 882 invasive Canadian case isolates collected between 2007 and 2014 by slide agglutination and PCR for serotyping, along with multilocus sequence typing (MLST).
Assayed isolates for β-lactamase production, sequenced the ftsI gene to detect PBP3 mutations, and performed disc diffusion across 14 antibiotics, with broth microdilution MIC testing on 316 isolates.
Non-typeable isolates comprised 54.6% of samples, while serotypeable isolates accounted for 45.4% (predominantly type a at 23.1%, type f at 10.8%, and type b at 8.3%).
Overall β-lactamase-mediated ampicillin resistance was 16.4%, increasing from 13.5% in 2007–2010 to 19.0% in 2011–2014, with significant PBP3 mutations detected in 14.6% of isolates (n=129).
Resistance markers were present in 52.5% of non-typeable strains with frequent trimethoprim/sulfamethoxazole resistance, whereas all isolates remained susceptible to fluoroquinolones and third-generation cephalosporins.

Cite This Study

Tsang et al. (2016) studied this question.

synapsesocial.com/papers/6aab57e04b88d88bf7bd0faf https://doi.org/10.1093/jac/dkw565

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Implication

Surveillance study reveals rising ampicillin resistance and penicillin-binding protein mutations in invasive Haemophilus influenzae, highlighting emerging drug resistance in non-typeable strains.

Antibiotic susceptibility and molecular analysis of invasiveHaemophilus influenzaein Canada, 2007 to 2014

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Implication

Key Points

Cite This Study

Antibiotic susceptibility and molecular analysis of invasiveHaemophilus influenzaein Canada, 2007 to 2014

Discussion

Member takes

Implication

Key Points

Cite This Study

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