Key result
Sensitive HPLC assay measures plasma normetanephrine and metanephrine up to 2000 pg without acetaminophen interference.
Why the study?
A method to measure unconjugated plasma normetanephrine and metanephrine without interference from acetaminophen was needed due to its widespread use.
Population
48 subjects (23 males, 25 females; mean age 49 +/- 14 years; 37 with hypertension) under resting conditions
Comparison
Measurement of unconjugated plasma NMN and MN with and without acetaminophen interference
Design
Analytical method development using HPLC with electrochemical detection
Authors
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May enable reliable pheochromocytoma screening despite acetaminophen; leaves open prospective validation before clinical use.
A sensitive HPLC assay for plasma metanephrines was developed that avoids interference from acetaminophen, facilitating accurate biochemical diagnosis of pheochromocytoma.
Roden et al. (2001) studied Hypertension (n=48). HPLC assay for unconjugated plasma metanephrines was evaluated on Assay linearity, precision, and interference by acetaminophen. A sensitive HPLC assay for plasma normetanephrine and metanephrine was developed that is linear up to 2000 pg and unaffected by simultaneous acetaminophen medication.
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