Key result
Ion exclusion chromatography matches Beckman EL-ISE for equine plasma TCO2 quantification within ~0.4 mM.
Why the study?
A suitable method for quantifying total carbon dioxide (TCO2) concentration in equine plasma to assess compliance with racing regulations was needed.
Ion exclusion chromatography is a suitable method for quantifying total carbon dioxide in equine plasma to ensure compliance with racing regulations.
May offer an alternative for equine plasma total CO2 quantification; leaves open larger validation before regulatory adoption.
Ion chromatography (IC), utilizing an ion exclusion column coupled to a conductivity detector (CD) via a post column reactor, had been evaluated for quantifying the Total Carbon dioxide (TCO 2 ) level in equine plasma.The concentrations obtained from the analysis of the ASE linearity set correlated with the results obtained when the measurement was done using a Beckman EL-ISE ® instrument (R 2 = 0.998).Equine blood was analyzed using both instruments and the averaged absolute difference for 30 samples was 0.4 mM.The IC method was considered suitable for use to determine the compliance of 36 mM available CO 2 per liter of plasma concentration threshold stated in the Malayan Racing Association's (MRA) Regulations.
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Chin‐Tong Tan (2013) studied Equine plasma TCO2 measurement (n=30). Ion Exclusion Chromatography vs. Beckman EL-ISE instrument was evaluated on Absolute difference in TCO2 concentration between methods (0.4 mM average absolute difference). Ion exclusion chromatography is a suitable alternative to the Beckman EL-ISE system for quantifying total carbon dioxide in equine plasma, demonstrating an average absolute difference of 0.4 mM.
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