Simultaneous Determination of Six Inorganic Anions in Animal Whole Blood Using Ion Chromatography With Suppressed Conductivity Detection

A sensitive and robust ion chromatography method with suppressed conductivity detection (IC‐CD) was developed and validated for the simultaneous quantification of six inorganic anions (fluoride, nitrite, bromide, nitrate, phosphate, and sulfate) in animal whole blood. The method employed a carbonate–bicarbonate eluent (4.5‐mM Na 2 CO 3 , 0.8‐mM NaHCO 3 ) at a flow of 1 mL/min and an anion‐exchange column (4 × 250 mm, Dionex IonPac AS23 RFIC) to achieve baseline resolution of all analytes within a 30‐min run. Sample preparation involved protein precipitation with ice‐cold methanol followed by centrifugation, dilution, and filtration. The method demonstrated excellent linearity ( R 2 = 0.9974–0.9998), with limits of detection (LODs) ranging from 0.27 to 2.96 mg/L. Accuracy was confirmed through recovery studies, yielding values between 99.4% and 118.2%. Intraday and interday precision, expressed as relative standard deviation (%RSD), was consistently below 10% for both peak areas and retention times. The developed IC‐CD protocol offers several advantages over existing techniques, including minimal sample volume, simplified sample preparation, rapid analysis, and high reproducibility. This method provides a valuable application tool for assessing ionic balance, nutritional status, and environmental exposure in animal health monitoring and toxicological studies.