Gel filtration of pregnancy urine on Sephadex G-100 separates two molecular species related to human chorionic gonadotrophin (HCG). One is a slightly retarded fraction which possesses biological activity but little or no immunological activity. The other is a highly retarded fraction which possesses immunological activity but no detectable biological activity. The highly retarded material could not be detected in pregnancy serum nor in extracts of first trimester placentas. A nonpregnant woman injected intravenously with purified HCG which displayed only slight retardation on the column produced urine which contained the highly retarded material. Experiments are described, the results of which suggest that the highly retarded material is a separate component of urine, probably generated by the kidney.
No takes yet. Share an insight, caveat, or question.
Matthies et al. (1971) studied this question.