Key result
Cardiac ATDPCs dose-dependently suppress T cell alloproliferation and modulate proinflammatory cytokine secretion in vitro.
Why the study?
Additional data on the potential immunogenicity and immunomodulatory properties of cardiac adipose tissue progenitor cells following implantation are needed.
Do cardiac ATDPCs suppress T cell alloproliferation similarly to UCBMSCs in an in vitro allostimulatory assay?
Do cardiac ATDPCs suppress T cell alloproliferation similarly to UCBMSCs in an in vitro allostimulatory assay?
Cardiac adipose tissue progenitor cells exhibit immunomodulatory properties in vitro, suppressing T cell alloproliferation and suggesting they may not trigger a harmful immune response if used for myocardial regeneration.
May support immune-compatible cardiac regeneration with ATDPCs; hypothesis-generating in vitro data leave clinical translation open.
Cell-based strategies to regenerate injured myocardial tissue have emerged over the past decade, but the optimum cell type is still under scrutiny. In this context, human adult epicardial fat surrounding the heart has been characterized as a reservoir of mesenchymal-like progenitor cells (cardiac ATDPCs) with potential clinical benefits. However, additional data on the possibility that these cells could trigger a deleterious immune response following implantation are needed. Thus, in the presented study, we took advantage of the well-established low immunogenicity of umbilical cord blood-derived mesenchymal stem cells (UCBMSCs) to comparatively assess the immunomodulatory properties of cardiac ATDPCs in an in vitro allostimulatory assay using allogeneic mature monocyte-derived dendritic cells (MDDCs). Similar to UCBMSCs, increasing amounts of seeded cardiac ATDPCs suppressed the alloproliferation of T cells in a dose-dependent manner. Secretion of proinflammatory cytokines (IL6, TNFα, and IFNγ) was also specifically modulated by the different numbers of cardiac ATDPCs cocultured. In summary, we show that cardiac ATDPCs abrogate T cell alloproliferation upon stimulation with allogeneic mature MDDCs, suggesting that they could further regulate a possible harmful immune response in vivo. Additionally, UCBMSCs can be considered as valuable tools to preclinically predict the immunogenicity of prospective regenerative cells.
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Perea‐Gil et al. (2015) studied Myocardial tissue injury. Cardiac adipose tissue progenitor cells (cardiac ATDPCs) vs. Umbilical cord blood-derived mesenchymal stem cells (UCBMSCs) was evaluated on T cell alloproliferation and secretion of proinflammatory cytokines. Cardiac adipose tissue progenitor cells suppressed T cell alloproliferation in a dose-dependent manner and modulated proinflammatory cytokine secretion in vitro.
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