Key result
Direct complement-fixation test detects IBV antigen and tracks antibody rise starting 6 days postinoculation.
Why the study?
A direct complement-fixation test was developed to detect infectious bronchitis virus antigen in eggs and antibodies in chicken serum to monitor infection and immune response.
A direct complement-fixation test can successfully detect infectious bronchitis virus antigen in eggs and measure antibody responses in chicken serum.
SUMMARY A direct complement-fixation (CF) test was used to measure infectious bronchitis virus (IBV) antigen in eggs, and antibodies in heat-inactivated chicken serum. The antigen was prepared from embryonating chicken eggs with the Beaudette strain of IBV. A suitable source of CF antigen was supernatant fluid from triturated chorio-allantoic membranes (CAM). The amnio-allantoic fluids were of low titer even after 20-fold concentration. The antibody response of individual chickens was followed. Serum collected from chickens before infection with the Massachusetts 41 strain of IBV did not react in the test. A sequential rise in CF titer was obtained beginning as early as 6 days postinoculation (PI). Antibody responses of individual chickens varied in onset, peak, and duration.
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W. W. Marquardt (1974) studied Infectious bronchitis virus (IBV) infection. Direct complement-fixation (CF) test was evaluated on Detection of IBV antigen and antibodies. A direct complement-fixation test successfully detected infectious bronchitis virus antigen in eggs and demonstrated a sequential rise in antibody titer in chicken serum beginning 6 days postinoculation.
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