Key result
Short-term hypoxia preserves adipose stromal cell viability and boosts superoxide dismutase activity by ~20%.
Why the study?
The response of perivascular adipose tissue-derived stromal cells to short-term hypoxic stress in a tissue-related oxygen microenvironment was not well characterized.
Tissue O2-adapted adipose-derived stromal cells are resistant to short-term hypoxic stress, maintaining viability and upregulating regenerative factors like VEGF-α and IL-6.
Tissue O2-adapted ASCs resist acute hypoxia with preserved viability and VEGF/IL-6 output; leaves open their regenerative utility in ischemic settings.
A microenvironment low in O2 ('physiological' hypoxia) governs the functions of perivascular multipotent mesenchymal stromal cells, defining their involvement in tissue physiological homeostasis and regenerative remodelling. Acute hypoxic stress is considered as one of the important factors inducing tissue damage. Here, we evaluate the influence of short-term hypoxia (1% O2 for 24 h) on perivascular adipose tissue-derived cells (ASCs) permanently expanded in tissue-related O2 (5%) microenvironment. After hypoxic exposure, ASCs retained high viability, stromal cell morphology and mesenchymal phenotype (CD73+, CD90+, CD105+ and CD45-). Mild oxidative damage was unveiled as elevation of reactive oxygen species and thiobarbituric acid-active products, while no reduction in the activity of the antioxidant enzymes catalase and glutathione peroxidase and a 20% statistically significant increase in superoxide dismutase activity was detected. Expression of hypoxia-inducible factor (HIF)-1α and HIF-3α isoforms was differently regulated. HIF-1α displayed transient up-regulation, with maximum levels 30 min after acute hypoxic exposure, while HIF-3α was significantly up-regulated after 24 h. Up-regulation of ERK7, MEK1 and c-fos, and down-regulation of MKK6, p53, CCNA2, CCNB1 and CCNB2 were observed after 24 h of oxygen deprivation. Acute hypoxic exposure did not affect the gene expression of other mitogen-activated protein kinases (MAPKs) and MAPK kinases, MAPK/ERK kinase-interacting proteins, MAPK-activated transcription factors and scaffolding proteins. Significant stimulation of vascular endothelial growth factor α and interleukin-6 production was detected in ASC-conditioned medium. Thus, tissue O2-adapted ASCs are resistant to hypoxic stress, which can ensure their effective involvement in the regeneration of tissue damage under significant oxygen deprivation.
No takes yet. Share an insight, caveat, or question.
Андреева et al. (2014) studied this question. Short-term hypoxia vs. Tissue-related O2 (5%) microenvironment was evaluated on Cell viability, morphology, and phenotype. Short-term hypoxia (1% O2 for 24 h) preserved high viability and mesenchymal phenotype in tissue O2-adapted adipose stromal cells, while inducing a 20% increase in superoxide dismutase activity.