Nutritional and regulatory roles of human serum in cultures of human granulopoietic cells

We proposed to develop a tissue culture medium supplement based on human serum that would support proliferation of human marrow cells and then to use it in an examination of human serum for factors that regulate colony formation.We examined various serum treatments ( heat, dialysis, ultrafiltration) and found that heat-treated human serum (58#{176}C for 120 mm) retained the ability to support proliferation and that this heat treatment eliminated the variability between different sera at concentrations 30%.