Key result
Monoclonal anti-DNA antibodies from B6-lpr/lpr mice exhibit diverse binding specificities mirroring autoimmune backgrounds.
Why the study?
The influence of genetic background on the diversity and specificity of lpr-induced anti-DNA autoantibody responses was unclear.
B6-lpr/lpr anti-DNA antibodies exhibit a diversity of binding reactions with a clonal repertoire similar to that of mice from autoimmune backgrounds.
The binding properties of B6-lpr/lpr anti-DNA monoclonal antibodies were characterized to evaluate the influence of genetic background on the diversity and specificity of lpr-induced autoantibody responses. Six anti-DNA antibodies were produced from fusions with B6-lpr/lpr mice, while another was obtained from a fusion with a B6-+/+ mouse immunized with lipopolysaccharide. Each antibody bound single-stranded DNA in preference to double-stranded DNA, with variation of over 300-fold in relative binding activities. In terms of binding to a panel of synthetic polynucleotides, each antibody exhibited a unique antigenic specificity. This binding, however, was not dependent on recognition of a unique base or sugar moiety, since individual antibodies bound polymers of dissimilar composition. These results suggest a diversity of binding reactions for B6-lpr/lpr anti-DNA antibodies, with a clonal repertoire similar to that of mice from autoimmune backgrounds.
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Warren et al. (1984) studied Autoimmune response. B6-lpr/lpr anti-DNA monoclonal antibodies vs. B6-+/+ mouse immunized with lipopolysaccharide was evaluated on Binding properties and antigenic specificity of anti-DNA antibodies. Monoclonal anti-DNA antibodies from B6-lpr/lpr mice exhibited a diversity of binding reactions and unique antigenic specificities, with a clonal repertoire similar to autoimmune backgrounds.
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