Laboratory study reveals distinct medium requirements for lipid yield and nervonic acid enrichment in Mortierella capitata, indicating potential for sustainable bioproduction.
Key Points
To optimize cultivation conditions, specifically glucose concentration and nitrogen source composition, for biomass, lipid accumulation, and nervonic acid enrichment in Mortierella capitata CBS 110633.
Cultivated Mortierella capitata CBS 110633 in shake-flask cultures using a stepwise evaluation approach.
Tested varying concentrations of glucose alongside different nitrogen sources, including yeast extract, peptone, and urea supplementation, across a 14-day period.
Increasing glucose concentration elevated lipid accumulation from approximately 4.8 g L−1 to 8.7 g L−1.
Cultivation with 60 g L−1 glucose, 4 g L−1 yeast extract, and urea supplementation for 14 days produced the highest nervonic acid enrichment at 9.5% of total fatty acids.
Medium containing 60 g L−1 glucose supplemented with 4 g L−1 yeast extract and 4 g L−1 peptone achieved the highest total lipid yield (8.7 g L−1) and estimated nervonic acid yield (504.7 mg L−1) at 14 days.