Key result
Dibutyryl cAMP upregulates three novel Na+ channel alpha subunit splice variants in cultured rat astrocytes.
Why the study?
The regulatory pathways controlling Na+ channel alpha subunit mRNA expression in spinal cord astrocytes compared to cerebral astrocytes and neuroblastoma cells were not fully understood.
Population
Cultured rat spinal cord and cerebral astrocytes and B50 and B104 neuroblastoma cells
Comparison
Exposure to 1 mM dibutyryl cAMP vs 1 microM A23187 vs no exposure
Design
Preclinical molecular biology study
Authors
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Hypothesis-generating for cAMP-regulated Na+ channel splicing in glia; leaves open in vivo or cardiac relevance.
Spinal cord astrocytes may have a unique regulatory pathway for Na+ channel mRNA expression involving cAMP.
Oh et al. (1998) studied this question. dibutyryl cAMP was evaluated on Expression of Na+ channel alpha subunit splice variants. Exposure to 1 mM dibutyryl cAMP upregulated the expression of three novel splice variants of the Na+ channel alpha subunit in cultured rat spinal cord astrocytes.
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