Key result
Unilateral ureteral obstruction cuts weight growth ~8% in high-fat diet mice but increases serum glucose.
Why the study?
A new animal model with kidney hypofunction induced by unilateral ureteral obstruction was developed to study factors related to obesity, dyslipidemia, and metabolic syndrome.
A kidney-hypofunction animal model induced by unilateral ureteral obstruction on a high-fat diet demonstrates susceptibility to metabolic syndrome.
Hypothesis-generating for metabolic effects of kidney hypofunction; does not support clinical practice changes.
Objectives: The objective of this study is to develop a new animal model with Kidney-hypofunction for Sasang Constitutional Medicine, especially for partial Soyangin(one of four constitution which has good digestive function and poor renal function) by Unilateral Ureteral Obstruction ,and to estimate the factor related to obesity, dyslipidemia, and metabolic syndrome.Methods: The C57BL/6J mice were divided into 3 groups : normal group, high fat diet(HFD) control group, and HFD group with Unilateral Ureteral Obstruction(UUO).Then, the HFD control group and the experimental group were fed with high fat diet for 6 weeks.Food intake and body weight were measured at regular time by week.After the final experiment, blood was gathered for bloodchemical examination and organs(liver, fatty tissue) were remoed, weighted, and mRNA was analyzed with real-time PCR.Results: The weight growth rate with High fat diet went down by 8.35% in experimental group and had similar FER with the normal group, while HFD control group had higher weight growth rate and FER than any other groups.Also The experimental group had lower triglyceride and LDL cholesterol rate and higher glucose rate in serum.and in mRNA expression, GLUT-9, the protein related to excretion of uric acid and metabolic syndrome, expressed lower rate than that of HFD control group.and IL-6, a kind of cytokine related to obesity and metabolic syndrome, expressed more than HFD control group.Conclusions: It was found that Kidney-hypofunction animal-experimental model is susceptible to metabolic syndrome.
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Kwak et al. (2018) studied Obesity and dyslipidemia in kidney-hypofunction (n=15). Unilateral Ureteral Obstruction (UUO) with High-Fat Diet vs. High-Fat Diet without UUO was evaluated on Weight growth rate. In a mouse model, unilateral ureteral obstruction combined with a high-fat diet reduced the weight growth rate by 8.35% and lowered triglyceride and LDL cholesterol levels compared to a high-fat diet alone, while increasing serum glucose and IL-6 expression.
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