Microbial Conversion with Cofactor Regeneration using Genetically Engineered Bacteria

In the synthesis of fine chemicals, biotransformations have been recognized as useful methods and applied for large-scale manufacturing. Especially, the recent development of recombinant DNA technology has greatly expanded the whole microbial cell processes for manufacturing fine chemicals. The whole-cell approaches have been applied not only to single conversion process but also to biotransformations requiring cofactor regeneration, such as reductions with NADH or NADPH and phosphorylations with ATP. The whole microbial cell conversion process with cofactor regeneration enables the production of complicated compounds, such as sugar nucleotides and oligosaccharides, on an industrial scale. 1 Introduction 2 NAD(P)