Key result
Transfection with cDNA libraries in an Epstein-Barr virus vector increases TNF receptor density ~150-fold.
Why the study?
An approach to isolate the cell-surface receptor for tumor necrosis factor (TNF) was needed by developing transfectants of human B-lymphoblastoid cells that overexpress the TNF receptor.
The study successfully developed a method to overexpress and characterize the TNF receptor in human B-lymphoblastoid cells, identifying it as a 68-kDa protein.
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Enables TNF receptor overexpression in B-lymphoblastoid cells; extends molecular tools but leaves open clinical translation.
Heller et al. (1990) studied this question. Transfection with cDNA libraries in EBO-pcD vector vs. Untransfected UC cells was evaluated on TNF receptor expression and binding characteristics. Transfection of human B-lymphoblastoid cells with cDNA libraries in an Epstein-Barr virus shuttle vector increased TNF receptor density by approximately 150-fold to about 150,000 per cell.
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