Key result
Replacing the 3CD region in severe Enterovirus 71 reduces viral replication and symptoms in mice.
Why the study?
The contribution of the 3CD region to the virulence and replication capacity of Enterovirus 71 strains was unclear.
Answers are grounded in this paper and cite their sources.
The 3CD region of Enterovirus 71 contributes to the virulence and temperature sensitivity of severe strains, suggesting a potential target for viral attenuation.
Enterovirus 71 is a neuroinvasive virus that is associated with severe neurological complications. We had earlier suggested that the replication capacity of a severe strain was higher than that of a mild strain. The recombinant 3CRV and 3CDRV virus strains were successfully rescued in our previous study. In the present study, we found no difference in virulence between 3CRV and severe strains. However, the capacity of replication and to cause cell injury of 3CDRV strain decreased in vitro, especially at 39.5 °C. Replacement of 3CD region in the severe strain led to milder symptoms, less body weight loss, and lower viral load in ICR mice. Histopathological findings indicated less severe injury in mice infected with 3CDRV strain. This study suggests that the 3CD region contributes to the attenuation of the severe strain, including its replication capacity and temperature sensitivity.
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Li et al. (2017) studied Enterovirus 71 infection. 3CD region replacement (3CDRV strain) vs. Severe strain was evaluated on Virulence, replication capacity, and cell injury. Replacement of the 3CD region in the severe Enterovirus 71 strain led to decreased replication capacity, milder symptoms, less body weight loss, and lower viral load in ICR mice.
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