Key result
Endothelial microparticle indices show acceptable visit-to-visit reproducibility in youth.
Why the study?
Reproducibility of endothelial microparticles enumeration among children and adolescents was not established.
Observational
Answers are grounded in this paper and cite their sources.
Endothelial microparticle enumeration indices, including total EMPs and percent-activated EMPs, are reproducible biomarkers in children and adolescents.
Aim: We assessed reproducibility of endothelial microparticles (EMPs) enumeration among youth. Methods & results: Four microparticle (MP) indices – total MP per microliter platelet free plasma (PFP), total EMPs per microliter PFP, percent activated EMPs and percent lactadherin positive (LACT[+]) of total EMPs – were measured at two visits (baseline and 7 ± 3 days follow-up) to determine reproducibility overall and by obesity status. We examined CD31+ or CD144+ with CD41-EMP events of size 0.3–1.0 μm. No statistically significant differences were observed between visits for any of the four MP indices. The within-participant and between-participant coefficient of variation was acceptable (range: 1.13–2.37) with good intraclass-correlation coefficient for all indices except total MP per microliter (range: 0.10–1.00). Conclusion: Total EMPs per microliter PFP, percent-activated EMPs and percent LACT(+) of total EMPs are reproducible among youth.
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Northrop et al. (2019) conducted an observational in Children and adolescents. Endothelial microparticles (EMPs) enumeration vs. Baseline vs 7 ± 3 days follow-up was evaluated on Reproducibility of four microparticle indices (CV range: 1.13-2.37; ICC range: 0.10-1.00, p=NS). Endothelial microparticle enumeration indices, including total EMPs, percent-activated EMPs, and percent LACT(+) EMPs, demonstrated acceptable reproducibility between visits among youth.
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