Identification of candidate genes in ischemic cardiomyopathy by gene expression omnibus database

Figures

• 

  Fig. 1. Heatmap of top 100 DEGs between ICM and healthy controls. Row and column represented DEGs and tissue samples, respectively. Color scale represented normalized expression levels of DEGs between ICM and healthy controls after log2 transformation. Red and green color were used to represent up- and downregulation in ICM, respectively

• 

  Fig. 2. Top 15 most significantly enriched GO terms and KEGG pathways of DEGs in ICM. a Biological process. b Cellular component. c Molecular function. d KEGG pathways. The x-axis shows -log FDR and y-axis shows GO terms or KEGG pathways

• 

  Fig. 3. The key pathway of DEGs enrichment. a The cardiac muscle contraction pathway. b The hypertrophic cardiomyopathy pathway. cThe arrhythmogenic right ventricular cardiomyopathy pathway. d The dilated cardiomyopathy pathway. The red rectangles were represented the components regulated by the DEGs that enriched in ICM

• 

  Fig. 4. The ICM-specific PPI network. Ellipses were used to represent nodes and lines were used to represent edges. Green represents a downward adjustment and red represents a downward adjustment. The black border indicates top20Up/Down

• 

  Fig. 5. ICM -specific transcriptional regulatory network. Ellipses and rhombus were used to represent nodes and lines were used to represent edges. The rhombus represented DEGs and the ellipses represented TFs. Green represents a downward adjustment and red represents a downward adjustment. The lines indicated TFs-DEGs pairs

• 

  Fig. 6. Validation of selected DEGs in GSE116250. a MYH6. b THBS4. c BCL6. d BLM. e IPO7. f SERPINA3. The x-axis shows healthy normal control (blue colour) and ICM (red colour) groups and y-axis shows a log2 transformation to the intensities. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001

• 

  Fig. 7. Validation DEG by qRT-PCR. The expression of DEG were detected by qRT-PCR assay. All of the assays were performed three times independently at least. Statistical significance was assessed by Student’s t-test. *P < 0.05