What question did this study set out to answer?

To clone and characterize the genetic determinants responsible for streptothricin resistance located on transposons Tn1825 and Tn1826.

January 1, 1988Journal of Basic Microbiology

Cloning and preliminary characterization of the streptothricin resistance determinants of the transposons Tn1825 and Tn1826

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Authors

ETE. TietzeRobert Koch Institute JBJean BrevetCentre National de la Recherche Scientifique HTH. TschäpeRobert Koch Institute

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Implication

Laboratory investigation reveals streptothricin acetyltransferase mediates antibiotic resistance in bacterial transposons, indicating a more complex genetic mechanism in Tn1825 than Tn1826.

Key Points

To clone and characterize the genetic determinants responsible for streptothricin resistance located on transposons Tn1825 and Tn1826.
Cloned streptothricin resistance determinants from Tn1825 and Tn1826 into the plasmid vector pUC8 and analyzed the physical structure of the recombinant plasmids.
Assessed bacterial host resistance by measuring minimal inhibitory concentrations of streptothricin and streptothricin acetyltransferase enzymatic activity.
Identified and analyzed the determinant-encoded proteins using an in vitro maxicell expression system.
Streptothricin acetyltransferase enzymatic activity mediates antibiotic resistance encoded by both transposons Tn1825 and Tn1826.
The resistance determinant of Tn1825 displays higher structural and functional complexity than that of Tn1826, indicating that additional genetic functions are required to express its full resistance phenotype.

Cite This Study

Tietze et al. (1988) studied this question.

synapsesocial.com/papers/6aacd047a37e8eb65f20f278 https://doi.org/10.1002/jobm.3620280116

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Implication

Laboratory investigation reveals streptothricin acetyltransferase mediates antibiotic resistance in bacterial transposons, indicating a more complex genetic mechanism in Tn1825 than Tn1826.

Cloning and preliminary characterization of the streptothricin resistance determinants of the transposons Tn1825 and Tn1826

Discussion

Member takes

Implication

Key Points

Cite This Study

Cloning and preliminary characterization of the streptothricin resistance determinants of the transposons Tn1825 and Tn1826

Discussion

Member takes

Implication

Key Points

Cite This Study

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