Key result
Inducing experimental allergic encephalomyelitis in guinea-pigs elevates spinal choline metabolites and depletes N-acetylated compounds.
Why the study?
The biochemical changes in spinal cord tissue, particularly betaine levels, during experimental allergic encephalomyelitis in Strain 13 guinea-pigs were not well characterized.
Does experimental allergic encephalomyelitis alter the levels of choline metabolites in the spinal cord of Strain 13 guinea-pigs?
Does experimental allergic encephalomyelitis alter the levels of choline metabolites in the spinal cord of Strain 13 guinea-pigs?
In a guinea-pig model of multiple sclerosis, spinal cord levels of betaine are elevated while N-acetylated compounds are depleted, which may aid in interpreting in vivo NMR spectra of MS patients.
Answers are grounded in this paper and cite their sources.
May aid MRS interpretation in MS; leaves open translation from guinea-pig EAE to human disease.
Chronic relapsing experimental allergic encephalomyelitis, an animal model of multiple sclerosis, was induced in Strain 13 guinea-pigs by subcutaneous injection of spinal cord homogenate and Freund's incomplete adjuvant supplemented with Mycobacterium tuberculosis. High resolution 1H NMR spectra of CNS tissue extracts indicated that the levels of choline metabolites, particularly betaine, were elevated in the spinal cord tissue, the principal site of lesion formation in this guinea-pig strain. The spectra also show that N-acetylated compounds are slightly depleted in the disease. The results are discussed in relation to the biochemical interpretation of NMR spectra obtained in vivo from patients with multiple sclerosis.
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Preece et al. (1993) studied Chronic relapsing experimental allergic encephalomyelitis (animal model of multiple sclerosis). Subcutaneous injection of spinal cord homogenate and Freund's incomplete adjuvant supplemented with Mycobacterium tuberculosis was evaluated on Levels of choline metabolites (betaine) and N-acetylated compounds in CNS tissue extracts. Induction of experimental allergic encephalomyelitis in Strain 13 guinea-pigs elevated levels of choline metabolites, particularly betaine, and slightly depleted N-acetylated compounds in the spinal cord.
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