Preparation of β-CD-DPPE-Dox Nanomedicine and Its’ Application as the Anticancer and Antitumor Drug

Figures

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  Figure 1. β-CD-DPPE molecules self-assemble into micelles which is used as drug delivery system to form nanomedicine for the treatment of tumor.

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  Figure 2. The synthesis illustration of terephthalic acid methyl ONSu ester.

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  Figure 3. The synthetic route of β-CD-NH-CO-C6H4-COOH.

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  Figure 4. The synthetic route of β-CD-DPPE molecule.

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  Figure 5. TEM image and size distribution of β-CD-DPPE micelles. (a) TEM image of micelles. (b) Size distribution of β-CD-DPPE micelles.

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  Figure 6. Release behavior of Dox from the β-CD-DPPE-Dox micelles at pH 5.0 and pH 7.4 separately in vitro at 25 °C and 37 °C separately.

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  Figure 7. MTT assay after 24 h incubation with HepG2 and MCF-7 cell. (a) Cytotoxicity of β-CD-DPPE on HepG2 cell. (b) Cytotoxicity of β-CD-DPPE on MCF-7 cell. (c) Anti-cancer activity of β-CD-DPPE-Dox liposome nanomedicines on HepG2 cancer cell. (d) Anti-cancer activity of β-CD-DPPE-Dox micelles nanomedicines on MCF-7 cancer cell. The significant level was set as P < 0.05.

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  Figure 8. LSCM images of HepG2 cell. (a) 10 μg·mL−1 β-CD-DPPE-Dox at 2 h; (b) 10 μg·mL−1 β-CD-DPPE-Dox at 8 h; (c) 10 μg·mL−1 β-CD-DPPE-Dox at 24 h; (d) 20 μg·mL−1 β-CD-DPPE-Dox at 2 h; (e) 20 μg·mL−1 β-CD-DPPE-Dox at 8 h; (f) 20 μg·mL−1 β-CD-DPPE-Dox at 24 h. (g) According to the mean fluorescence intensity (See Fig. S9) acquired the histogram with concentration and time changed.

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  Figure 9. The therapeutic effect of β-CD-DPPE-Dox micelles nanomedicine in vivo. (a) Tumor before injected the PBS. (b) Tumor before injected the β-CD-DPPE micelles. (c) Tumor before injected the β-CD-DPPE-Dox micelles nanomedicine. (d) Tumor injected the PBS after 12 days. (e) Tumor injected the β-CD-DPPE micelles after 12 days; (f) Tumor injected the β-CD-DPPE-Dox micelles after 12 days. (g) The tumor volume change with the time increase. (h) The weight change with the time increase. (i) Tissue section of tumor was injected PBS after 12 days. (j) Tissue section of tumor was injected β-CD-DPPE micelles after 12 days. (k) Tissue section of tumor was injected β-CD-DPPE-Dox micelles nanomedicine after 12 days.