Key result
CRISPR-Cas systems enable rapid and highly sensitive point-of-care detection of nucleic acid biomarkers.
Why the study?
Highly infectious illnesses caused by pathogens pose severe public health threats and require improved rapid diagnostic methods.
CRISPR-Cas systems provide a robust and programmable platform for rapid point-of-care diagnostics.
CRISPR-Cas enables rapid multiplex pathogen detection; leaves open routine clinical adoption pending prospective validation studies.
Highly infectious illnesses caused by pathogens constitute severe threats to public health and lead to global economic loss. The use of robust and programmable clustered regularly interspaced short palindromic repeat and CRISPR-associated protein (CRISPR-Cas) systems, repurposed from genome-engineering applications has markedly improved traditional nucleic acid detection for precise identification, independently enabling rapid diagnostics of multiplex biomarker with genetic and mutation related to tumors, and microbial pathogens. In this review, we delineate the utility of the current CRISPR-Cas enzyme as biosensors by which these effector toolkits achieve recognition, signaling amplification, and finally, accurate detection. Additionally, we discuss the details of the dominance and hurdles related to expanding this revolutionary technology into an effective and convenient contraption crucial for improving the rational redesign to CRISPR/Cas biosensing. Overall, this review provides an insight into the current status of rapid and POC diagnostic systems by CRISPR/Cas tools.
No takes yet. Share an insight, caveat, or question.
Liu et al. (2022) conducted a review in Infectious diseases and tumors (pathogen and biomarker detection). CRISPR/Cas-based biosensors vs. Traditional nucleic acid detection methods (e.g., PCR, ELISA) was evaluated. CRISPR-Cas systems, including Cas9, Cas12, Cas13, and Cas14, offer highly sensitive, specific, and rapid programmable biosensing platforms for the point-of-care detection of nucleic acid biomarkers.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: