Key result
Ultra-rapid PCR1100 detects RSV in under 20 minutes with accuracy matching conventional RT-PCR.
Why the study?
Rapid and accurate detection methods for human orthopneumovirus (RSV) comparable in speed to rapid antigen tests were needed to improve point-of-care testing.
Does ultra-rapid real-time RT-PCR using the PCR1100 device provide comparable sensitivity and specificity to conventional RT-PCR for detecting RSV in less time?
Does ultra-rapid real-time RT-PCR using the PCR1100 device provide comparable sensitivity and specificity to conventional RT-PCR for detecting RSV in less time?
An ultra-rapid real-time RT-PCR method using the PCR1100 device can detect RSV in under 25 minutes with high sensitivity and specificity, offering a potential point-of-care alternative to rapid antigen tests.
Human orthopneumovirus, also known as the respiratory syncytial virus (RSV), is a leading cause of respiratory tract infections in children worldwide. The World Health Organization has taken steps toward establishing a global surveillance system for RSV, based on the global influenza surveillance and response system initiated in 2015. The US Centers for Disease Control and Prevention (CDC) has developed a genetic detection method based on real-time reverse transcription polymerase chain reaction (RT-PCR), which is used in global RSV surveillance. In Japan, immunoassay-based rapid antigen detection kits are widely used for the detection of RSV. In this study, an ultra-rapid real-time RT-PCR method for the rapid detection of RSV was developed using the PCR1100 device based on the US CDC assay in order to detect RSV in comparable time to rapid test kits. The ultra-rapid real-time RT-PCR could detect RSV viral RNA in less than 20 min while maintaining sensitivity and specificity comparable to conventional real-time RT-PCR using large installed instruments. Furthermore, combining ultra-rapid real-time RT-PCR with the M1 Sample Prep kit reduced the total working time for the detection of RSV from clinical specimen to less than 25 min, suggesting this method could be used for point-of-care RSV testing.
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Shirato et al. (2020) studied Respiratory syncytial virus (RSV) infection (n=84). Ultra-rapid real-time RT-PCR using PCR1100 vs. Conventional real-time RT-PCR was evaluated on Detection of RSV viral RNA (sensitivity and specificity). The ultra-rapid real-time RT-PCR using the PCR1100 device detected RSV viral RNA in less than 20 minutes with sensitivity and specificity comparable to conventional real-time RT-PCR.
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