Evaluation of Grapevines (Vitis Vinifera L.) Genetic Diversity by Using Microsatellite Markers

The allelic characteristics of the DNA microsatellite loci (VVS2, ZAG62, VVMD7, VVMD27, VVMD5, VVMD25, VVMD28, ZAG79, VVMD32) of 80 grapes varieties and forms were used to deduct the main indicators of genetic diversity. The values of these indicators fluctuated within: the number of alleles N a – from 8 (VVMD25) to 18 (VVMD28); the effective number of alleles N e – from 4.90 (VVMD32) to 8.57 (ZAG79); expected heterozygosity H e – 0.744 (VVMD25) to 0.883 (ZAG79); the observed heterozygosity H o from 0,3823 (VVMD25) to 0,925 (ZAG79), the probability of occurrence of null alleles r – from -0,009 (VVMD32) to -0,051 (VVMD27), probability of identity PI – from 0,024 (VVMD32) to 0,109 (VVMD25), Wrights index F – from -0,026 (VVMD5) to -0.115. 720 genotypes of microsatellite loci were identified, among them 91 are homozygous, 629 are heterozygous. The highest homozygosity among all studied loci has been determined for highly polymorphic locus VVMD32, that may indicate the domination of certain alleles in the region. Comparison of the parameters of genetic diversity determined by us with the results of other authors showed a general similarity, and in some cases – excess of values.