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Commercially available ELISA tests for detecting Phytophthora and Pythium species were used to detect propagules of these genera in water samples. Samples were filtered through 0.45-μm filters to concentrate propagules before extraction. Heating filter residues to 100 C for 5 min yielded extracts that were equal or superior in reactivity to those obtained by liquid nitrogen disruption. As few as 30-40 zoospore cysts were detected in filter pad extracts. Detection sensitivity (propagules per liter) depends on the volume of water that can be passed through the filters (.)
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Mohammed S. Ali-Shtayeh (1991) studied this question.
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