Nitrate reductase in crude extracts of phytoplankton from cultures and from natural populations from the Oslofjord (Norway) was assayed at different incubation temperatures. The temperature optimum of the enzyme was 10°–15°C and 15°–20°C for cultures and about 10°C for natural plankton. The utility of the nitrate reductase assay as an ecological tool is greatly diminished unless performed at the temperature optimum.
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S. Kristiansen (1983) studied this question.
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