A New Class of Arylamine Oxygenases: Evidence that p‐Aminobenzoate N‐Oxygenase (AurF) is a Di‐iron Enzyme and Further Mechanistic Studies

Alternative route. The p-aminobenzoate N-oxygenase (AurF) from the aureothin biosynthetic pathway in Streptomyces thioluteus is a novel di-iron enzyme. Rather than through the previously proposed mechanism, AurF in fact catalyzes the conversion of substrate p-aminobenzoate to p-nitrobenzoate through at least three consecutive reactions: two monooxygenation steps (a and c) and one dehydrogenation step (b).