Key result
Resveratrol inhibits proliferation and induces death in rat cardiac fibroblasts while sparing cardiomyocytes.
Why the study?
The potential cardioprotective effects of resveratrol on adult rat cardiac fibroblasts, myofibroblasts, and cardiomyocytes were investigated due to unclear differential cellular responses.
Resveratrol selectively inhibits proliferation and induces cell death in cardiac fibroblasts and myofibroblasts without affecting cardiomyocyte survival.
Resveratrol effects in rat cardiac cells remain preliminary; leaves open any human cardioprotective role.
In this study, we tested the potential cardioprotective effects of the phytoalexin resveratrol (Rsv) on primary adult rat cardiac fibroblasts (CF), myofibroblasts (MF) and cardiomyocytes. Adult rat CF and cardiomyocytes were isolated from male 10-week old Sprague-Dawley rats, cultured for either 24 h (cardiomyocytes) or 48 h (CF) before treatments. To isolate MF, CF were trypsinized after 48 h in culture, seeded in fresh plates and cultured for 24 h prior to treatment. All three cells were then treated for a further 24 h with a range of Rsv doses. In CF and MF, cell proliferation, viability, apoptosis assays were performed with or without Rsv treatment for 24 h. In cardiomyocytes, cell viability and apoptosis assay were performed 24 h after treatment. In separate experiments, CF was pre-incubated with estrogen, tamoxifen and fulvestrant for 30 min prior to Rsv treatment. Rsv treatment decreased proliferation of both fibroblasts and myofibroblasts. Rsv treatment also increased the proportion of dead CF and MF in a dose dependent manner. However, treatment with Rsv did not induce cell death in adult cardiomyocytes. There was an increase in the percentage of cells with condensed nuclei with Rsv treatment in both CF and MF, but not in cardiomyocytes. Treatment with estrogen, tamoxifen and fulvestrant alone or in combination with Rsv did not have any additional effects on CF survival. Our results demonstrate that treatment with Rsv can inhibit cell proliferation and induce cell death in rat CF and MF, while not affecting cardiomyocyte survival. We also demonstrated that the induction of cell death in CF with Rsv treatment was independent of estrogen receptor alpha (ERα) signaling.
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Louis et al. (2019) studied this question. Resveratrol vs. without resveratrol treatment was evaluated on cell proliferation, viability, and apoptosis. Resveratrol treatment inhibited cell proliferation and induced cell death in rat cardiac fibroblasts and myofibroblasts, while not affecting cardiomyocyte survival.
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