What question did this study set out to answer?

To determine whether resveratrol directly inhibits cardiac fibroblast growth, proliferation, and phenotypic differentiation into hypersecretory myofibroblasts.

October 21, 2004AJP Heart and Circulatory Physiology

Inhibition of cardiac fibroblast proliferation and myofibroblast differentiation by resveratrol

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Why the study?

Does resveratrol inhibit proliferation and differentiation in cardiac fibroblasts?

Population

Cardiac fibroblasts (CFs)

Comparison

Resveratrol (RES) 5-25 microM vs Untreated CFs or CFs stimulated with ANG II…

Design

Preclinical

Authors

EOErik R. OlsonImmusoft (United States)JNJennifer E. NaugleUnited States Department of Justice XZXiaojin ZhangJiangsu Province Hospital

Discussion

Member takes

Implication

Resveratrol may inhibit cardiac fibroblast activation in vitro; hypothesis-generating for anti-fibrotic effects in vivo.

Key Points

To determine whether resveratrol directly inhibits cardiac fibroblast growth, proliferation, and phenotypic differentiation into hypersecretory myofibroblasts.
Cultured cardiac fibroblasts were pretreated with resveratrol (5–25 µM) before stimulation with angiotensin II, epidermal growth factor, or transforming growth factor-beta.
Cell growth, proliferation, and intracellular signaling were quantified using [³H]leucine and [³H]thymidine incorporation assays alongside Western blot analyses of ERK1/2 and Akt phosphorylation.
Myofibroblast differentiation was evaluated by measuring alpha-smooth muscle actin expression and stress fiber organization.
Resveratrol (5–25 µM) concentration-dependently inhibited basal and angiotensin II–induced cardiac fibroblast proliferation and protein synthesis via attenuation of ERK1/2 phosphorylation, without altering Akt phosphorylation.
Resveratrol pretreatment attenuated epidermal growth factor–stimulated ERK phosphorylation to a degree comparable to its inhibition of angiotensin II signaling.
Resveratrol suppressed both angiotensin II– and transforming growth factor-beta–induced differentiation, markedly reducing alpha-smooth muscle actin expression and stress fiber assembly.

Structured PICO

Does resveratrol inhibit proliferation and differentiation in cardiac fibroblasts?

Population

Cardiac fibroblasts (CFs)

Intervention

Resveratrol (RES) 5-25 microM

Comparator

Untreated CFs or CFs stimulated with ANG II, EGF, or TGF-beta alone

Outcome

CF proliferation (measured by [(3)H]leucine and [(3)H]thymidine incorporation) and differentiation to myofibroblast phenotype (alpha-smooth muscle actin expression and stress fiber organization)surrogate

Resveratrol demonstrates anti-fibrotic properties in vitro by inhibiting cardiac fibroblast proliferation and myofibroblast differentiation.

Cite This Study

Olson et al. (2004) studied this question.

synapsesocial.com/papers/6a73501d1dc3df6e344e53be https://doi.org/10.1152/ajpheart.00763.2004

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Inhibition of cardiac fibroblast proliferation and myofibroblast differentiation by resveratrol

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Inhibition of cardiac fibroblast proliferation and myofibroblast differentiation by resveratrol

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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