Key result
Obese female mice develop higher antibody responses and better influenza protection than males.
Why the study?
Sex differences in influenza vaccine-induced immunity and protection during obesity are limited and not well understood.
Does biological sex affect influenza vaccine-induced immunity and protection in a mouse model of obesity?
Does biological sex affect influenza vaccine-induced immunity and protection in a mouse model of obesity?
In a mouse model of obesity, females exhibit greater influenza vaccine-induced immunity and protection than males, highlighting the importance of considering biological sex and obesity status in vaccine design.
Sex differences in obese mice suggest considering biological sex in vaccine research; leaves open translation to human influenza immunization.
Introduction Obesity is increasing globally, and it negatively impacts influenza vaccine efficacy. Although sex differences in influenza vaccine responses are studied in non-obese hosts, studies investigating sex differences in influenza vaccine-induced immunity and protection during obesity are limited. Materials and methods Using the C57BL/6J mouse model of high-fat diet (HFD)-induced obesity or low-fat diet controls, we investigated sex differences in influenza vaccine-induced immunity and protection during obesity. Male and female mice with or without obesity were vaccinated intramuscularly twice at a 3-week interval with an inactivated 2009 H1N1 influenza A virus (IAV) vaccine. At 35 days post-vaccination (dpv), antibody responses in plasma and B- and T-cell responses in spleen and bone marrow were quantified. At 42 dpv, mice were intranasally challenged with a drift variant of the H1N1 IAV, and disease severity was assessed by monitoring the change in body mass up to 21 days post-challenge (dpc). Subsets of mice were euthanized at 3 dpc to determine pulmonary virus replication (TCID 50 assay), histopathology (H&E staining), and cytokine/chemokine responses (multiplex ELISA). Results Female mice, irrespective of diet and obesity status, developed higher antibody responses and were better protected compared to males. Vaccinated males with obesity mounted the poorest antibody responses, experienced a more severe disease, were unable to clear replicating virus from the lungs effectively, and demonstrated heightened pulmonary inflammation. Despite these differences, splenic B- and T-cell frequencies were comparable, suggesting the inefficiency of B cells to produce antibodies in males but not in females with obesity. Discussion Our findings suggest that sex differences is observed in influenza vaccine-induced immunity and protection during obesity, where males are more severely affected. These findings highlight the importance of considering biological sex and obesity status in influenza vaccine design and testing.
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Wolfe et al. (2025) studied Influenza infection during obesity. Inactivated 2009 H1N1 influenza A virus (IAV) vaccine vs. Male vs female mice; obese vs non-obese was evaluated on Antibody responses, disease severity, pulmonary virus replication, and inflammation. Female mice with obesity developed higher antibody responses and were better protected against influenza challenge compared to males, who experienced more severe disease and pulmonary inflammation.
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