What question did this study set out to answer?

To identify the structural and functional determinants within A-Myb and c-Myb transcription factors that govern their unique endogenous target gene specificities.

July 1, 2004Journal of Biological ChemistryOpen Access

Positive and Negative Determinants of Target Gene Specificity in Myb Transcription Factors

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Authors

WLWanli LeiLovelace Respiratory Research Institute JRJohn J. RushtonUniversity of New Mexico LDLisa M. DavisNavidea Biopharmaceuticals (United States)

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Implication

Experimental assays reveal discrete activation elements governing distinct target gene selection by Myb transcription factors across cell types, highlighting context-dependent gene regulation.

Key Points

To identify the structural and functional determinants within A-Myb and c-Myb transcription factors that govern their unique endogenous target gene specificities.
Measured endogenous gene expression changes following individual expression of A-Myb and c-Myb across distinct cell types.
Conducted deletion and domain-swap mutagenesis within the transcription factors to isolate specific regulatory elements required for target gene activation.
A-Myb and c-Myb activated distinct, nearly nonoverlapping sets of endogenous genes in a cell-type-dependent manner despite sharing a conserved DNA-binding domain.
Identified discrete positive and negative sequence motifs in the transcriptional activation domains required for regulating specific genes, including DHRS2, DSIPI, and mim-1, which standard reporter gene assays failed to detect.

Cite This Study

Lei et al. (2004) studied this question.

synapsesocial.com/papers/6aade6d0ffd142a6660dcb4c https://doi.org/10.1074/jbc.m403133200

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Implication

Experimental assays reveal discrete activation elements governing distinct target gene selection by Myb transcription factors across cell types, highlighting context-dependent gene regulation.

Positive and Negative Determinants of Target Gene Specificity in Myb Transcription Factors

Discussion

Member takes

Implication

Key Points

Cite This Study

Positive and Negative Determinants of Target Gene Specificity in Myb Transcription Factors

Discussion

Member takes

Implication

Key Points

Cite This Study

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