What question did this study set out to answer?

To establish a dual-cut CRISPR/Cas9 strategy for simultaneous genome and high-resolution epigenome editing using exogenous methylated donor DNA.

November 18, 2019BMC BiologyOpen Access

Concurrent genome and epigenome editing by CRISPR-mediated sequence replacement

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Authors

JAJes AlexanderUniversity of California, San Francisco GFGregory M. FindlayThe Francis Crick Institute MKMartin KircherUniversity of Lübeck

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Implication

Experimental study demonstrates functional gene silencing using methylated DNA sequence replacement in human cells, highlighting that promoter methylation directly silences transcription.

Key Points

To establish a dual-cut CRISPR/Cas9 strategy for simultaneous genome and high-resolution epigenome editing using exogenous methylated donor DNA.
Introduced dual CRISPR/Cas9 double-strand breaks flanking a target sequence in the HPRT1 promoter.
Supplied in vitro methylated exogenous DNA to mediate sequence replacement through non-homologous end joining repair.
Replacement of the target locus with heavily methylated CpG island alleles induced functional transcriptional silencing of the HPRT1 gene.
Single-event concurrent genome and epigenome editing was successfully achieved, despite limited overall replacement efficiency.

Cite This Study

Alexander et al. (2019) studied this question.

synapsesocial.com/papers/6aadfa07ad4280f693b8b3e5 https://doi.org/10.1186/s12915-019-0711-z

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Implication

Experimental study demonstrates functional gene silencing using methylated DNA sequence replacement in human cells, highlighting that promoter methylation directly silences transcription.

Concurrent genome and epigenome editing by CRISPR-mediated sequence replacement

Discussion

Member takes

Implication

Key Points

Cite This Study

Concurrent genome and epigenome editing by CRISPR-mediated sequence replacement

Discussion

Member takes

Implication

Key Points

Cite This Study

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