Key result
HPLC method measures plasma vitamin K1 with interday variation under ~15%.
Why the study?
A selective and sensitive method for routine measurement of vitamin K1 in small plasma samples was needed for clinical pharmacological studies.
The described HPLC method is robust and convenient for routine measurement of vitamin K1 in small volumes of human plasma.
Answers are grounded in this paper and cite their sources.
Facilitates small-volume vitamin K1 assays in labs; leaves open its role in guiding supplementation decisions.
BACKGROUND: A selective and sensitive high-performance liquid chromatographic method with fluorescence detection after postcolumn reduction is described for the routine measurement of vitamin K1 in plasma samples of 100 µL of volume. METHODS: Liquid-liquid extraction was used for the sample preparation with vitamin K2 as an internal standard. For the chromatographic separation, a standard C18 column was applied. The calibration range used was from 2 to 500 ng/mL. RESULTS: At the low level (5 ng/mL), a coefficient of variation of 14.9% was observed interday in the quality control samples, whereas at the intermediate (50 ng/mL) and high (200 ng/mL) levels such of 6.8% and 8.7% were found. CONCLUSIONS: The method has proven robust and convenient for the analysis of samples from clinical pharmacological studies.
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Meineke et al. (2013) studied this question. High-performance liquid chromatographic method with fluorescence detection was evaluated on Coefficient of variation in quality control samples. A high-performance liquid chromatographic method for measuring vitamin K1 in 100 µL plasma samples demonstrated interday coefficients of variation between 6.8% and 14.9% across different levels.
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