Key result
Membrane-bound Dlk1 promotes myocyte hypertrophy and fusion, while soluble Dlk1 inhibits myotube formation.
Why the study?
The roles of different Dlk1 isoforms in myogenesis have not been investigated.
Dlk1 isoforms have distinctive and opposing effects on myogenesis, with membrane-bound Dlk1 promoting and soluble Dlk1 inhibiting muscle development.
Answers are grounded in this paper and cite their sources.
May guide isoform-targeted myogenesis research; leaves open clinical translation pending in vivo validation.
Delta-like 1 homolog (Dlk1) is important in myogenesis. However, the roles of different Dlk1 isoforms have not been investigated. In C2C12 cell lines producing different Dlk1 isoforms, membrane-bound Dlk1 promoted the hypertrophic phenotype and a higher fusion rate, whereas soluble Dlk1 inhibited myotube formation. Inversed expression patterns of genes related to myogenic differentiation further support these phenotypic changes. In addition, temporal expression and balance between the Dlk1 isoforms have a regulatory role in myogenesis in vivo. Collectively, Dlk1 isoforms have distinctive effects on myogenesis, and its regulation during myogenesis is critical for normal muscle development.
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Shin et al. (2014) studied Myogenesis. Membrane-bound Dlk1 vs. Soluble Dlk1 was evaluated on Myotube formation and fusion rate. Membrane-bound Dlk1 promoted a hypertrophic phenotype and higher fusion rate in C2C12 cells, whereas soluble Dlk1 inhibited myotube formation.
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