Key result
HUMSCs harboring ACE2 reduce rat acute lung injury vs either alone by curbing inflammation and permeability.
Why the study?
Acute lung injury (ALI) has a high incidence and mortality rate with no effective therapeutic method available.
Does treatment with human umbilical cord mesenchymal stem cells harboring ACE2 improve acute lung injury in a bleomycin-induced rat model?
Does treatment with human umbilical cord mesenchymal stem cells harboring ACE2 improve acute lung injury in a bleomycin-induced rat model?
Mesenchymal stem cells engineered to express ACE2 provide superior attenuation of acute lung injury in a rat model compared to stem cells or ACE2 alone.
BACKGROUND: Acute lung injury (ALI) is a high incidence disease with no effective therapeutic method (mortality rate > 40%). The aim of this study was to find a new and effective therapeutic method for ALI. METHODS: After the isolation of human umbilical cord mesenchymal stem cells (HUMSCs) from cesarean fetus, we transfected the HUMSCs with Lenti-ACE2 (angiotensin-converting enzyme 2) viral particles. Then we evaluated the therapeutic effect of HUMSCs harboring ACE2 on ALI, which induced by bleomycin (BLM) in rat model. RESULTS: Our results showed that HUMSCs harboring ACE2 could attenuate ALI degree through reducing pulmonary inflammatory infiltration and degree of vascular permeability, repressing the mRNA level of pro-inflammatory cytokines, activating the mRNA level of anti-inflammatory cytokines and ACE2. Besides, results also demonstrated that HUMSCs harboring ACE2 gene had higher therapeutic effects to ALI than the single factor of HUMSCs or ACE2. CONCLUSIONS: This research provided clues for the development of effective therapeutic methods to ALI using stem cell transplantation and gene therapy.
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Gao et al. (2014) studied Acute lung injury (ALI). Human umbilical cord mesenchymal stem cells (HUMSCs) harboring ACE2 vs. HUMSCs alone or ACE2 alone was evaluated on ALI degree (pulmonary inflammatory infiltration, vascular permeability, cytokine mRNA levels). HUMSCs harboring ACE2 attenuated acute lung injury in rats more effectively than HUMSCs or ACE2 alone by reducing inflammation and vascular permeability.
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