Key result
Novel Drosophila actin-related protein maps to the X chromosome and shows expression throughout development.
Why the study?
Actin-related proteins in Drosophila are divergent from conventional actins, and the identification of the most divergent ARP may represent a new isotype.
Key points are not available for this paper at this time.
Design
Molecular identification and characterization of a divergent ARP gene in Drosophila
The actin-related proteins (ARPs) have primary sequence homology to actin, have no homology to other proteins and, unlike the conventional actins, are clearly divergent. We have identified an ARP in Drosophila that has approximately 30% amino acid identity to most actins, making it the most divergent yet reported. It is also quite divergent from all other ARP sequences. When the Drosophila ARP is aligned with actin it contains sequence insertions, as is the case with all other ARPs. The unique location of the insertions, as well as its overall divergence, indicates it may represent a new isotype. Only one gene was detected by hybridization to both genomic DNA and polytene chromosomes; the location of the gene is 13E on the X chromosome. A transcript of 1350 bases was detected at all stages of development. This transcript was relatively abundant during early embryogenesis, decreasing during the later stages of embryogenesis and increasing again in larvae and adults.
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Frankel et al. (1994) studied this question. A novel, highly divergent actin-related protein (ARP) was identified in Drosophila, mapped to the X chromosome at 13E, and expressed throughout all developmental stages.
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