Key result
Next-generation sequencing drives ultra high-throughput full-length genome sequencing of human RNA viral pathogens.
Why the study?
There is an increasing effort to produce full length viral genome sequences to better understand viral adaptation and improve molecular diagnosis of emerging viral infections.
Next generation sequencing offers an ultra high-throughput strategy for full length viral genome sequencing to monitor viral evolution and improve molecular diagnosis.
Advances prospects for real-time viral surveillance; leaves open standardized NGS protocols for clinical diagnostics.
In the face of numerous emerging and re-emerging viral threats, large-scale genome sequencing efforts are underway to monitor viral evolution in real-time. To fully appreciate the mechanisms of viral adaptation and evolution, and to also develop reagents and resources for a better molecular diagnosis of emerging and re-emerging viral infections, there has been an increasing effort toward producing full length viral genome sequences. To date, high-throughput platforms have been developed using traditional Sanger-based sequencing and there are currently prospects to apply next generation sequencing methods to develop an ultra high-throughput strategy for viral genome sequencing and analysis.
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Djikeng et al. (2008) conducted a review in Human RNA viral pathogens. Full length genome sequencing was evaluated. Next-generation sequencing methods are being applied to develop ultra high-throughput strategies for full-length genome sequencing of human RNA viral pathogens.
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