Molecular detection ofJasmine potyvirusassociated with yellow mosaic symptoms onJasminum sambacL. in India

Association of Jasmine potyvirus with the yellow mosaic disease of Jasminum sambac L. was detected by reverse transcription-polymerase chain reaction (RT-PCR) using potyvirus coat protein (CP) gene-specific primers and confirmed by Southern hybridisation test with CP gene-specific probe prepared from a known potyvirus. The RT-PCR amplicons were cloned, sequenced and 616 nucleotide sequence data deposited in GenBank database (JN807771). The sequence data of jasmine virus isolate shared highest 94% identities during BLASTn, high amino acid sequence homology in multiple alignment analysis and closest phylogenetic relationship with Taiwanese isolate of J. potyvirus T. infecting J. sambac; therefore, the virus under study was identified as an isolate of J. potyvirus.