The Effect of Darapladib Therapy for the Expression of Lp-PLA2 in Dyslipidemia and Type 2 Diabetes Mellitus Atherosclerosis Model

Atherosclerosis is the main cause of mortality and morbidity globally.Lipoprotein-associated phospholipase A 2 (Lp-PLA 2 ) activity is suspected to have a significant role in atherosclerosis.50 Sprague-Dawley Rats were divided into five groups: normal, dyslipidemia, Type 2 diabetes mellitus (T2DM), dyslipidemia with darapladib administration and T2DM with darapladib administration.These groups were divided into two serial times: 8 and 16 weeks.mRNA Lp-PLA 2 was measured from blood and aortic tissue extraction.Aortic tissue Lp-PLA 2 was measured by immunofluorescence.Lp-PLA 2 expression in aortic tissue was consistently increased in dyslipidemia and T2DM.The expression of Lp-PLA 2 enzymatic was significantly suppressed (p < 0.05) with the administration of darapladib especially in 8 weeks groups in both dyslipidemia and T2DM.The administration of darapladib in dyslipidemia and T2DM didn't significantly suppress the expression of mRNA Lp-PLA 2 in blood and aortic tissue.The failure of genetic expression suppression of Lp-PLA 2 was found in both 8 weeks and 16 weeks groups.The expression of Lp-PLA 2 protein also showed an inclined difference between dyslipidemia and T2DM.These results showed that administration of darapladib significantly decreased Lp-PLA 2 protein but prone to increase the expression of mRNA Lp-PLA 2 in blood and aortic tissue in dyslipidemia and T2DM model.