Key result
Low-dose trigonelline protects H9c2 cells from apoptosis via caspase downregulation, but higher doses induce necrosis.
Why the study?
The mechanisms by which trigonelline reduces hydrogen peroxide induced oxidative stress and toxicity in cardiomyocytes were not fully elucidated.
Does trigonelline protect H9c2 cells from hydrogen peroxide-induced apoptosis?
Does trigonelline protect H9c2 cells from hydrogen peroxide-induced apoptosis?
Trigonelline protects H9c2 cardiomyocytes from oxidative stress-induced apoptosis by regulating caspase and Bcl-2 family gene expression, suggesting potential utility in oxidative stress-mediated cardiovascular diseases.
Should not yet change practice in oxidative cardiac injury; hypothesis-generating for trigonelline cardioprotection, requires in vivo validation.
OBJECTIVE: To elucidate the key parameters associated with hydrogen peroxide induced oxidative stress and investigates the mechanism of trigonelline (TG) for reducing the H2O2 induced toxicity in H9c2 cells. METHODS: Cytotoxicity and antioxidant activity of TG was assessed by EZ-CYTOX kit. RNA extraction and cDNA synthesized according to the kit manufacture protocol. Apoptosis was measured by the Flowcytometry, general PCR and qPCR. RESULTS: It was found that the TG significantly rescued the morphology of the H9c2 cells. Treatment of cells with TG attenuated H2O2 induced cell deaths and improved the antioxidant activity. In addition, TG regulated the apoptotic gene caspase-3, caspase-9 and anti-apoptotic gene Bcl-2, Bcl-XL during H2O2 induced oxidative stress in H9c2 cells. These results were comparable with quercetin treatment. For evident, flow cytometer results also confirmed the TG significantly reduced the H2O2 induced necrosis and apoptosis in H9c2 cells. However, further increment of TG concentration against H2O2 could induce the necrosis and apoptosis along with H2O2. CONCLUSIONS: It is suggested that less than 125 μ M of TG could protect the cells from H2O2 induced cell damage by down regulating the caspases and up regulating the Bcl-2 and Bcl-XL expression. Therefore, we suggest the trigonelline could be useful for treatment of oxidative stress mediated cardiovascular diseases in future.
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Soundharrajan et al. (2015) studied Hydrogen peroxide induced oxidative stress and apoptosis. Trigonelline vs. Hydrogen peroxide alone / Quercetin was evaluated on Cytotoxicity, antioxidant activity, and apoptosis. Trigonelline (<125 μM) protected H9c2 cells from H2O2-induced apoptosis by downregulating caspases and upregulating Bcl-2 and Bcl-XL expression, though higher concentrations induced necrosis.
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