Key result
TbAQP2 depletion impairs growth and volume recovery while triple AQP knockdown allows compensatory glycerol uptake.
Why the study?
The specific roles and cellular localization of the three aquaglyceroporins in bloodstream form Trypanosoma brucei and their contribution to osmoregulation and glycerol transport were not fully characterized.
Population
Bloodstream form Trypanosoma brucei
Comparison
Knockdown of AQP1, AQP2, AQP3, and simultaneous knockdown of all 3 versus non-knockdown parasites
Design
Inducible inheritable double-stranded RNA interference study
Authors
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TbAQP2 may represent a drug target in T. brucei; leaves open therapeutic translation to human trypanosomiasis.
This study elucidates the specific roles and localizations of aquaglyceroporins in Trypanosoma brucei, highlighting TbAQP2's critical role in osmoregulation and cell growth.
Bassarak et al. (2011) studied Trypanosoma brucei. RNA interference knockdown of aquaglyceroporins (AQP1-3) was evaluated on Cell growth, regulatory volume recovery, and glycerol uptake. Depletion of TbAQP2 negatively impacted cell growth and regulatory volume recovery, while simultaneous knockdown of all 3 AQPs allowed glycerol uptake substitution via a putative transporter.
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