Target DNA detection by solid-state nanopores using gold nanoparticle probes

This is the authors' abstract. We don't add key points for this paper.

For target DNA detection with nanopores, the labeling of target DNA with large probes and the detection of its passage through a nanopore were examined. We labeled the target DNA with two gold nanoparticles (AuNPs). The surface of each AuNP was modified with a different single-stranded DNA (ssDNA) molecule that contained a complementary sequence to a different part of the target DNA so that the two AuNPs could capture one target DNA. The observed ionic current blockade when the AuNPs-labeled target DNA passed through a nanopore was significantly larger than that when nonlabeled DNA or a single AuNP probe passed through a nanopore. This result indicated that the presence of the target DNA in solution can be detected through nanopore measurements with two AuNP probes.